Microbiology 311 Lab Report

Rebekah Worley February 21, 2012 Mitchell surgical incision 4 Biol 311 Staining and Identifying Unknown bacterium Introduction The microbiology testing ground up to this render has been apply to teach the students how to target and give away bacteria. There are several types of spying through which the bacteria can be set based on the semblance and knead. The staining methods used in the lab are gravitational constant Staining, Capsule Staining, Endospore Staining, and vinegarish Fast staining. One of the most substantive method of staining is the Gram Staining, as it is highly dependent (McCarthy, 25).In the specific look into that was done, Gram Staining was used and the bacteria that was found was purplish and round (cocci) shaped. with this the bacteria was identified as staphylococcus epidermis. Material and Methods The first note to identifying the bacteria was to heat fix it to the slue. The materials used were a seacoast, water, a Bunsen Burner, bibulous paper and clothes pin. The unknown bacteria was in a vial in solid form. The travel on page 19 and 20 of the Customized Biol 311 full general Microbiology Laboratory manual were followed to heat fix the bacteria.After this gram staining was used to identify the unknown bacteria. The materials used for gram staining include the splay the was heat fixed, bibulous paper, crystallisation violet, distilled water, Grams iodine, 95% ethyl alcohol, safranin, oil and a microscope. The steps on page 26 of the Customized Biol 311 usual Microbiology Laboratory Manual were used to stain the bacteria. Several alters were made in the procedure. The crystal violet was on the slide for 1 minute alternatively than 20 seconds. The decolorizing step was used with alcohol for 10 seconds rather than 20 seconds.The only other change was that the safranin was on the slide for 1 minute instead of the recommended 20 seconds. The slide was put below the microscope at 1000x gush using oil immersion . Results When looking under the microscope the bacteria was found to be purple and cocci shaped. Because of the specific color and shape of the bacteria it was easily identifiable as Staphylococcus epidermis. From this it is seen that only a Gram stain was necessary to identify the bacteria. handling From this try out it is seen that bacteria is easily identified when stained correctly.Going through the procedure with the true is vital, and when done right the bacteria is pull ahead and concise. When the bacteria was stained in this experiment the color was difficult to determine at first. After exploring the bacteria on the slide it was seen to be mainly purple. If the staining had not been done properly it would have been a lot more difficult to signalize between bacteria. This was an important thing to direct because staining is so vital in identifying unknown bacterium. Works Cited McCarthy, Charlotte M and Harold Benson. Customized Biol 311 General Microbiology Laborator y Manual. 2nd. ed. New York. McGraw-Hill 2002 Print.